Bioinformatics strategies for lipidomics analysis.
Take your Lipidomics research to new levels. SCIEX offers a suite of powerful instrumentation, workflows, and software for successful lipidomics research, identification, and quantitation of lipid species, including all-in-one solutions to get you up and running fast.
Modification of the host lipidome via secreted enzymes is an integral, but often overlooked aspect of bacterial pathogenesis. In the current era of prevalent antibiotic resistance, knowledge regarding critical host pathogen lipid interactions has the potential for use in developing novel antibacterial agents. While most studies to date on this matter have focused on specific lipids, or select.
Lipidomic methodologies have developed such that determination in lipid species content of cells and tissues is increasingly achievable. Adoption of these methods is highlighting the physiological.
Dysregulation of lipid metabolism is responsible for pathologies of human diseases including metabolic diseases. Recent advances in lipidomics analysis allow for the targeted and untargeted identification of lipid species and for their quantification in normal and diseased conditions. Herein, this review provides a brief introduction to lipidomics, highlights its application to characterize.
Lipid-related pathways, or portions of pathways are displayed in tabular format with lipid structures and related information organized in columns. Pathways may also be viewed in an enzymatic reaction format, where substrate and product are displayed in separate rows, with an intervening row showing the gene information for the enzyme catalyzing that step.
Lipidomics is the comprehensive analysis of molecular lipid species, including their quantitation and metabolic pathways. The huge diversity of native lipids and their modifications make lipidomic analyses challenging. The method of choice for sensitive detection and quantitation of molecular lipid species is mass spectrometry, either by direct infusion (shotgun lipidomics) or coupled with.
For lipid analysis it has proven very successful as an orthogonal separation technique by allowing lipids to be fractioned in the gas phase by the dipole moment on their head groups. As the device is located at the front of the mass spectrometer, it helps eliminate isobaric overlap often encountered during any lipid analysis, whether applying accurate mass or nominal mass spectrometry.